column purification Search Results


pcr purification kit  (Jena Bioscience)
95
Jena Bioscience pcr purification kit
Pcr Purification Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna purification spin columns  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc dna purification spin columns
Dna Purification Spin Columns, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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rnasep prep rna purification column  (ADS Biotec)
93
ADS Biotec rnasep prep rna purification column
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Rnasep Prep Rna Purification Column, supplied by ADS Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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rnasep semipreparative column  (ADS Biotec)
92
ADS Biotec rnasep semipreparative column
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Rnasep Semipreparative Column, supplied by ADS Biotec, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
rnasep semipreparative column - by Bioz Stars, 2026-04
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nucleospin plasmid  (MACHEREY NAGEL)
96
MACHEREY NAGEL nucleospin plasmid
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Nucleospin Plasmid, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleospin plasmid - by Bioz Stars, 2026-04
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nucleospin plasmid easypure  (MACHEREY NAGEL)
94
MACHEREY NAGEL nucleospin plasmid easypure
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Nucleospin Plasmid Easypure, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleospin rna plus kit  (MACHEREY NAGEL)
95
MACHEREY NAGEL nucleospin rna plus kit
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Nucleospin Rna Plus Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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nucleospin rna plus xs kit  (MACHEREY NAGEL)
94
MACHEREY NAGEL nucleospin rna plus xs kit
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Nucleospin Rna Plus Xs Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleospin rna plus xs kit - by Bioz Stars, 2026-04
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protino ni nta  (MACHEREY NAGEL)
94
MACHEREY NAGEL protino ni nta
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Protino Ni Nta, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleospin rna columns  (MACHEREY NAGEL)
94
MACHEREY NAGEL nucleospin rna columns
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Nucleospin Rna Columns, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protino gst 4b column  (MACHEREY NAGEL)
94
MACHEREY NAGEL protino gst 4b column
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Protino Gst 4b Column, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna purification kit  (Jena Bioscience)
96
Jena Bioscience rna purification kit
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Rna Purification Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna purification kit/product/Jena Bioscience
Average 96 stars, based on 1 article reviews
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Image Search Results


Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). Co-purification of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated RNA bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.

Journal: Nucleic Acids Research

Article Title: 2′- O -Methylation of the second transcribed nucleotide within the mRNA 5′ cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion

doi: 10.1093/nar/gkac722

Figure Lengend Snippet: Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). Co-purification of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated RNA bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.

Article Snippet: The crude mRNAs were purified using NucleoSpin RNA Clean-up XS (740903, Macherey-Nagel). mRNAs were HPLC-purified using an RNASep Prep RNA Purification Column (ADS Biotec) at 55°C, a linear gradient of buffer B (0.1 M triethylammonium acetate pH 7.0 and 50% acetonitrile) from 17.5% to 25.8% in buffer A (0.1 M triethylammonium acetate pH 7.0) over 20 min and a flow rate of 0.9 ml/min. mRNAs from collected fractions were recovered by isopropanol precipitation.

Techniques: Methylation, Concentration Assay, Copurification, Western Blot, Incubation